Nucleotide sequence of a developmentally regulated Dictyostelium discoideum endogenous plasmid gene and 5' flanking region
نویسندگان
چکیده
منابع مشابه
A developmentally regulated kinesin-related motor protein from Dictyostelium discoideum.
The cellular slime mold Dictyostelium discoideum is an attractive system for studying the roles of microtubule-based motility in cell development and differentiation. In this work, we report the first molecular characterization of kinesin-related proteins (KRPs) in Dictyostelium. A PCR-based strategy was used to isolate DNA fragments encoding six KRPs, several of which are induced during the de...
متن کاملDevelopmentally regulated, carbohydrate-binding protein in Dictyostelium discoideum.
A carbohydrate-binding protein assayed by its ability to agglutinate formalinized sheep erythrocytes is synthesized between 3 and 9 hr after Dictyostelium discoideum cells are deprived of food, as the cells become cohesive. Agglutination of erythrocytes by this protein was inhibited by N-acetyl-D-galactosamine, D-galactose, and L-fucose, but other monosaccharides had little or no effect. The pr...
متن کاملNucleotide sequence of the 5'-flanking region of the murine ornithine decarboxylase gene.
We have sequenced a 1674basepair Pst I fragment ( 1 6 5 8 to +16, relative to the transcription start site) from the 5"-f1ank1ng region of the mouse ornithine decarboxylase (ODC) gene, Isolated from a genomtc library of BALB/c mice (generously provided by Dr. J. V. Ravetch). Previously, Brabant et al. ( 1 ) and Katz and Kahane ( 2 ) have reported sequences for the 5'-flank1ng region of this gen...
متن کاملmRNA stabilization controls the expression of a class of developmentally regulated genes in Dictyostelium discoideum.
During the development of Dictyostelium discoideum, several thousand new mRNA species appear in the cytoplasm after the cells have formed stable aggregates. Here we show that six of these late mRNAs, corresponding to six clones randomly chosen from a genomic library, are synthesized from the very beginning of development at a rate comparable to that observed late in development but that transcr...
متن کاملEffects of a post-translational modification mutation on different developmentally regulated glycosidases in Dictyostelium discoideum.
We have studied the effect of a post-translational modification mutation upon four developmentally regulated glycosidases of Dictyostelium discoideum. The presence of the modA mutation affects the intracellular level of these multimeric enzymes differently. The level of alpha-glucosidase is unaffected in the modA mutant. The mutant cell contains only a very small fraction of the wild type beta-...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1988
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/16.22.10914